deoxynucleotide triphosphate dntp (TaKaRa)
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TaKaRa
deoxynucleotide triphosphate dntp
Deoxynucleotide Triphosphate Dntp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 8855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deoxynucleotide+triphosphates/dNTP+Mixture/pmc13039009-120-16-33
Average 96 stars, based on 8855 article reviews
Deoxynucleotide Triphosphate Dntp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 8855 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deoxynucleotide+triphosphates/dNTP+Mixture/pmc13039009-120-16-33
Average 96 stars, based on 8855 article reviews
deoxynucleotide triphosphate dntp - by Bioz Stars,
2026-09
96/100 stars
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Reverse Transcription:Article Title: Pyrroloquinoline Quinone Alleviates Tris(1,3-Dichloro-2-Propyl) Phosphate-Induced Damage During Mouse Oocyte Maturation. Article Snippet: .. Cytoplasmic fractions containing RNA were transferred into 45 μL of reverse transcription mixture, which was composed of 200 U/μL SuperScript II Reverse Transcriptase (18064071, Invitrogen, Carlsbad, CA, USA), 10 μL template switch oligo primer, 40 U/μL RNase inhibitor, 10 μM 6-base pair (bp) barcode primer, 10 mM Article Title: Single-cell multi-omics analysis decodes molecular characteristics of sheep oocyte fate in vivo maturation Article Snippet: .. Single-cell RNA library construction and sequencing Firstly, the RNA-containing cytoplasm was transferred into 4.5 μL reverse transcription mix, which contained 200 U /μL SuperScript II Reverse Transcriptase (18064071, Invitrogen), 10 μL template switch oligo primer, 40 U /μL RNase inhibitor, 10 μM 6-base pair (bp) barcode primer, 10 mM Article Title: Pyrroloquinoline Quinone Alleviates Tris(1,3-Dichloro-2-Propyl) Phosphate-Induced Damage During Mouse Oocyte Maturation Article Snippet: .. Cytoplasmic fractions containing RNA were transferred into 45 μL of reverse transcription mixture, which was composed of 200 U/μL SuperScript II Reverse Transcriptase (18064071, Invitrogen, Carlsbad, CA, USA), 10 μL template switch oligo primer, 40 U/μL RNase inhibitor, 10 μM 6-base pair (bp) barcode primer, 10 mM Single Cell:Article Title: Single-cell multi-omics analysis decodes molecular characteristics of sheep oocyte fate in vivo maturation Article Snippet: .. Single-cell RNA library construction and sequencing Firstly, the RNA-containing cytoplasm was transferred into 4.5 μL reverse transcription mix, which contained 200 U /μL SuperScript II Reverse Transcriptase (18064071, Invitrogen), 10 μL template switch oligo primer, 40 U /μL RNase inhibitor, 10 μM 6-base pair (bp) barcode primer, 10 mM Sequencing:Article Title: Single-cell multi-omics analysis decodes molecular characteristics of sheep oocyte fate in vivo maturation Article Snippet: .. Single-cell RNA library construction and sequencing Firstly, the RNA-containing cytoplasm was transferred into 4.5 μL reverse transcription mix, which contained 200 U /μL SuperScript II Reverse Transcriptase (18064071, Invitrogen), 10 μL template switch oligo primer, 40 U /μL RNase inhibitor, 10 μM 6-base pair (bp) barcode primer, 10 mM Article Title: A multi-kingdom genetic barcoding system for precise clone isolation Article Snippet: .. An EGFP coding sequence was then amplified from pLV-eGFP (Addgene, no. 36083) in 25 separate 50 μl PCR reactions, each containing 1 ng μl −1 of pLV-eGFP template plasmid, 1.25 μl of 20 μM SI#679 oligonucleotide pool as the forward primer, 1.25 μl of 20 μM SI#680 as the common reverse primer, 0.5 μl of Phusion High-fidelity DNA Polymerase (NEB, no. M0530), 10 μl of 5× Phusion HF Buffer (NEB, no. B0518S) and 5 μl of 2.5 mM Article Title: A multi-kingdom genetic barcoding system for precise clone isolation. Article Snippet: .. An EGFP coding sequence was then amplified from pLV-eGFP (Addgene, no. 36083) in 25 separate 50 μl PCR reactions, each containing 1 ng μl−1 of pLV-eGFP template plasmid, 1.25 μl of 20 μM SI#679 oligonucleotide pool as the forward primer, 1.25 μl of 20 μM SI#680 as the common reverse primer, 0.5 μl of Phusion High-fidelity DNA Polymerase (NEB, no. M0530), 10 μl of 5× Phusion HF Buffer (NEB, no. B0518S) and 5 μl of 2.5 mM Polymerase Chain Reaction:Article Title: Sequential CRISPR screening reveals partial NatB inhibition as a strategy to mitigate alpha-synuclein levels in human neurons. Article Snippet: The primers also specifically amplify genome- integrated parts of the lentiGuide- puro (Addgene #52963) backbone. .. PCR reactions were performed in a volume of 100- μl reactions containing 5 μg of gDNA, 0.5 μM of each primer (forward and reverse), 2.5 mM of Article Title: A multi-kingdom genetic barcoding system for precise clone isolation Article Snippet: .. An EGFP coding sequence was then amplified from pLV-eGFP (Addgene, no. 36083) in 25 separate 50 μl PCR reactions, each containing 1 ng μl −1 of pLV-eGFP template plasmid, 1.25 μl of 20 μM SI#679 oligonucleotide pool as the forward primer, 1.25 μl of 20 μM SI#680 as the common reverse primer, 0.5 μl of Phusion High-fidelity DNA Polymerase (NEB, no. M0530), 10 μl of 5× Phusion HF Buffer (NEB, no. B0518S) and 5 μl of 2.5 mM Article Title: Application of two-dimensional polymerase chain reaction to detect four types of microorganisms in feces for assisted diagnosis of IBD. Article Snippet: Background: The incidence of inflammatory bowel disease (IBD) continues to increase annually, accounting for about 6.8 million cases in 2017 worldwide.. However, there is currently no gold standard for the diagnosis of IBD.. Methods: A method for the detection of four microorganisms in feces by two-dimensional polymerase chain reaction (2D-PCR) has been developed. Article Title: A multi-kingdom genetic barcoding system for precise clone isolation. Article Snippet: .. An EGFP coding sequence was then amplified from pLV-eGFP (Addgene, no. 36083) in 25 separate 50 μl PCR reactions, each containing 1 ng μl−1 of pLV-eGFP template plasmid, 1.25 μl of 20 μM SI#679 oligonucleotide pool as the forward primer, 1.25 μl of 20 μM SI#680 as the common reverse primer, 0.5 μl of Phusion High-fidelity DNA Polymerase (NEB, no. M0530), 10 μl of 5× Phusion HF Buffer (NEB, no. B0518S) and 5 μl of 2.5 mM Article Title: Sequential CRISPR screening reveals partial NatB inhibition as a strategy to mitigate alpha-synuclein levels in human neurons Article Snippet: The primers also specifically amplify genome-integrated parts of the lentiGuide-puro (Addgene #52963) backbone. .. PCR reactions were performed in a volume of 100-μl reactions containing 5 μg of gDNA, 0.5 μM of each primer (forward and reverse), 2.5 mM of Amplification:Article Title: A multi-kingdom genetic barcoding system for precise clone isolation Article Snippet: .. An EGFP coding sequence was then amplified from pLV-eGFP (Addgene, no. 36083) in 25 separate 50 μl PCR reactions, each containing 1 ng μl −1 of pLV-eGFP template plasmid, 1.25 μl of 20 μM SI#679 oligonucleotide pool as the forward primer, 1.25 μl of 20 μM SI#680 as the common reverse primer, 0.5 μl of Phusion High-fidelity DNA Polymerase (NEB, no. M0530), 10 μl of 5× Phusion HF Buffer (NEB, no. B0518S) and 5 μl of 2.5 mM Article Title: A multi-kingdom genetic barcoding system for precise clone isolation. Article Snippet: .. An EGFP coding sequence was then amplified from pLV-eGFP (Addgene, no. 36083) in 25 separate 50 μl PCR reactions, each containing 1 ng μl−1 of pLV-eGFP template plasmid, 1.25 μl of 20 μM SI#679 oligonucleotide pool as the forward primer, 1.25 μl of 20 μM SI#680 as the common reverse primer, 0.5 μl of Phusion High-fidelity DNA Polymerase (NEB, no. M0530), 10 μl of 5× Phusion HF Buffer (NEB, no. B0518S) and 5 μl of 2.5 mM Plasmid Preparation:Article Title: A multi-kingdom genetic barcoding system for precise clone isolation Article Snippet: .. An EGFP coding sequence was then amplified from pLV-eGFP (Addgene, no. 36083) in 25 separate 50 μl PCR reactions, each containing 1 ng μl −1 of pLV-eGFP template plasmid, 1.25 μl of 20 μM SI#679 oligonucleotide pool as the forward primer, 1.25 μl of 20 μM SI#680 as the common reverse primer, 0.5 μl of Phusion High-fidelity DNA Polymerase (NEB, no. M0530), 10 μl of 5× Phusion HF Buffer (NEB, no. B0518S) and 5 μl of 2.5 mM Article Title: A multi-kingdom genetic barcoding system for precise clone isolation. Article Snippet: .. An EGFP coding sequence was then amplified from pLV-eGFP (Addgene, no. 36083) in 25 separate 50 μl PCR reactions, each containing 1 ng μl−1 of pLV-eGFP template plasmid, 1.25 μl of 20 μM SI#679 oligonucleotide pool as the forward primer, 1.25 μl of 20 μM SI#680 as the common reverse primer, 0.5 μl of Phusion High-fidelity DNA Polymerase (NEB, no. M0530), 10 μl of 5× Phusion HF Buffer (NEB, no. B0518S) and 5 μl of 2.5 mM Labeling:Article Title: Application of two-dimensional polymerase chain reaction to detect four types of microorganisms in feces for assisted diagnosis of IBD. Article Snippet: Background: The incidence of inflammatory bowel disease (IBD) continues to increase annually, accounting for about 6.8 million cases in 2017 worldwide.. However, there is currently no gold standard for the diagnosis of IBD.. Methods: A method for the detection of four microorganisms in feces by two-dimensional polymerase chain reaction (2D-PCR) has been developed. |